RNA Droplets

Rolling droplets

When a rigid circular cylinder or sphere is placed on a rough inclined plane it will roll down the plane. When the experiment is repeated with a rigid cube it will slide down the plane. If the object is deformable a variety of motions become possible; the motion of elastic bodies and fluid drops depends on the interfacial energies of the materials, the roughness of the interfaces, the size of t...

PREBIOTIC CHEMISTRY Rapid RNA Exchange in Aqueous Two-Phase System and Coacervate Droplets

Compartmentalization in a prebiotic setting is an important aspect of early cell formation and is crucial for the development of an artificial protocell system that effectively couples genotype and phenotype. Aqueous two-phase systems (ATPSs) and complex coacervates are phase separation phenomena that lead to the selective partitioning of biomolecules and have recently been proposed as membrane...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

Lipid droplets

OK, they are cute, but why should a developmental biologist care about them? Onset of calcification early in ontogeny coupled with gradual, progressive morphological development permits the recognition of growth sequences for over a hundred trilobite species. Striking variation in the number of trunk segments at maturity across ontogeny and phylogeny, coupled with the curious pattern of segment...

Enhancement of RNA Interference Effect in P19 EC Cells by an RNA-dependent RNA Polymerase

Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...